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Effect of NNMT inhibitor on adipocyte differentiation quantitated with oil red O staining 3T3-L1 cells were cultured in 60 mm diameter dishes (8.410 4 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 M dexamethasone, 10 g/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)

Moreover, Researchers have used it in cultured human myoblasts, but no established human therapeutic safety or efficacy profile exists

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In the 12-week randomised controlled trial documented in the obesity pharmacotherapy literature, the 1 mg/day group achieved statistically meaningful separation from placebo

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